Composite
Part:BBa_K3431051:Design
Designed by: Jian-An Pan, Cheng-Yang Ma, Yi-Ching Chen, Shen-Lin Chen, Huan-Jui Chang Group: iGEM20_CSMU_Taiwan (2020-10-18)
Banana_ToeholdSwitch-Regulated Invertase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1441
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1212
Illegal BamHI site found at 1342
Illegal XhoI site found at 1413 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1013
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We included T7 promoter and terminator to ensure correct functioning of this device in PURExpress in vitro protein synthesis kit.
Source
We obtained the sequence of invertase from BBa_K3431000, and synthesized the construct through IDT.