Composite

Part:BBa_K3431051:Design

Designed by: Jian-An Pan, Cheng-Yang Ma, Yi-Ching Chen, Shen-Lin Chen, Huan-Jui Chang   Group: iGEM20_CSMU_Taiwan   (2020-10-18)


Banana_ToeholdSwitch-Regulated Invertase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1441
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1212
    Illegal BamHI site found at 1342
    Illegal XhoI site found at 1413
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1013
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We included T7 promoter and terminator to ensure correct functioning of this device in PURExpress in vitro protein synthesis kit.


Source

We obtained the sequence of invertase from BBa_K3431000, and synthesized the construct through IDT.

References